WebA flexible assembly strategy has been developed for the synthesis of Leishmania donovani GPI anchors that bear a clickable alkyne tag. This strategy is based on the use of the 2 … WebMay 6, 2016 · Further, the alkyne label on RNA provides a convenient handle for the posttranscriptional chemical functionalization with a variety of biophysical tags (fluorescent, affinity, amino acid and sugar ...
Benchmarking Cleavable Biotin Tags for Peptide-Centric …
WebJul 21, 2024 · A flexible assembly strategy has been developed for the synthesis of Leishmania donovani GPI anchors that bear a clickable alkyne tag. This strategy is … WebOct 8, 2008 · Abstract. We demonstrated the metal−organic framework bearing the azide group in the organic linkers and in situ click reactions with some small alkynes. The XRPD patterns indicated that the click reaction proceeded without any decomposition of the original MOF network. Controlling the organic linkers and incorporation of the azide groups ... happy anniversary to my husband poem
Using Clickable NAD+ Analogs to Label Substrate Proteins of PARPs
WebTerminal Alkynes. Labeling Reagents; Biotinylation Reagents; Cleavable Biotin Probes; Dual Labeling Biotin Probes; Fluorescent Alkynes; Kits and Auxiliary Reagents. Click-&-Go IsoTaG Kit and Reagents; Click-&-Go® … WebApr 4, 2012 · We have developed clickable active site-directed photoaffinity probes for γ-secretase which incorporate a photoreactive benzophenone group and an alkyne handle for subsequent click chemistry mediated conjugation with azide-linked reporter tags for visualization (e.g., TAMRA-azide) or enrichment (e.g., biotin-azide) of labeled proteins. WebJul 23, 2024 · An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne functionality at their C-terminus via the intein-mediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody–intein–chitin binding domain (CBD) fusion protein in E. coli SHuffle ... chains of infectious serrations