WebJul 25, 2024 · Ideally, this fluorogen cofactor should (1) emit no fluorescence in its free state, (2) bind the RNA aptamer with high affinity (sub-micromolar or even sub-nanomolar KD ), (3) produce strong fluorescence upon binding, and (4) be photostable. All these properties rely on both partners as demonstrated below. WebApr 13, 2024 · The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has …
Ex Vivo and In Vivo Imaging and Biodistribution of Aptamers …
WebWe recently developed a convenient and versatile method that uses a gene-specific RNA aptamer and a fluorescent probe to enable spatiotemporal imaging of endogenous mRNAs in living cells. The method was validated by live-cell imaging of the endogenous mRNA of β-actin. The new RNA-imaging technology might be useful for live-cell … WebSep 8, 2024 · This study reports a novel aptamer selection method based on microscale electrophoretic filtration. Aptamers are versatile materials that recognize specific targets and are attractive for their applications in biosensors, diagnosis, and therapy. However, their practical applications remain scarce due to issues with conventional selection methods, … greek theater berkeley parking
Biosensors Free Full-Text Recent Advances in …
WebLight-up RNA aptamers or Fluorescent light-up aptamers (FLAPs) are a genetically-encoded RNA imaging platform. They are designed to bind specific fluorogenic dyes that 'light-up' only in the bound state. This property of 'fluorogenicity' means that fluorescence can be 'switched on' upon RNA expression. Light-up aptamers can be thought of as the ... WebJul 16, 2015 · Time-resolved microspectrofluorimetry and fluorescence microscopy imaging—two complementary fluorescence techniques—provide important information about the intracellular distribution, level of uptake and binding/interactions inside living cell of the labeled molecule of interest. They were employed to monitor the “fate” of AS1411 … flower delivery poplar bluff mo