Photocleavable 意味
WebMay 31, 2024 · Photocleavable molecules can enable the light-dependent modulation of biomolecular activities with high spatiotemporal precision. We have previously reported a … Webconsists of the incorporation of the photocleavable fluorescent nucleotide into the DNA, detection of the fluorescent signal, and photocleavage of the fluorophore. The entire process was re-peated to identify 12 continuous bases in the DNA template. These results demonstrate that photocleavable fluorescent nucleotide
Photocleavable 意味
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WebOct 1, 2024 · O-nitrobenzyls and coumarins have specific photosensitivity and can be photoisomerized under suitable light exposure. Importantly, compared to other photosensitive molecules, o-nitrobenzyls and coumarins, which have multiple functional groups, can serve as photocleavable linkers to crosslink polymers, biomolecules and … WebApr 26, 2012 · Photo-cleavable (PC) modifications contain a photolabile functional group that is cleavable by UV light of specific wavelength (300–350 nm). The PC Spacer is a 10 …
WebMeaning of photocleavable. What does photocleavable mean? Information and translations of photocleavable in the most comprehensive dictionary definitions resource on the web. WebPC Biotin Azide is an azide-activated photocleavable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to an azide group through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5 ...
WebAbstract. Photocleavable molecules can enable the light-dependent modulation of biomolecular activities with high spatiotemporal precision. We have previously reported a … http://genelink.com/newsite/products/mod_detail.asp?modid=133
WebAbstract. To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase ...
WebJan 21, 2003 · Three single-stranded DNA molecules of different lengths were synthesized and characterized, each containing a fluorescent dye (6-carboxyfluorescein) connected to the 5' end via a photocleavable 2-nitrobenzyl linker and a biotin moiety at the 3' end. UV irradiation (lambda approximately 340 nm) of s … high speed home printerWebJan 15, 2012 · Photocleavable groups must be carefully selected if they are to be used in conjunction with photo-reactive groups (eg. diazirine, phenylazide and benzophenone) which are activated in near-UV light. Acid sensitive linkages are very versatile especially since they can be cleaved in mildly acidic medium. A wide panel of acid labile functions was ... high speed homogenizer d-500WebAug 1, 1995 · Abstract. While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm ... high speed hotspot softwareWebNov 4, 2024 · Taken together, this study introduces photocleavable flipper probes 3 and 4, which release small molecule mechanophores to image order and tension of any … high speed hole sawWebPC Amino C6 (Photocleavable) PC Linker (photocleavable) is a non-nucleosidic molety that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 … high speed host controllerWebJan 6, 2003 · The 2-nitrobenzyl group is widely used in organic synthesis as a protecting group and a cleavable linker because of its high photocleavage efficiency by near-UV light irradiation (1–4).A cleavage quantum yield of 0.49–0.63 has been reported in the literature for 1-(2-nitrophenyl)ethyl phosphate esters ().This unique property of the photocleavable … how many days is 1778 hoursWebunique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphora-midite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry (8, 9). how many days is 1787 hours