WebSingle-Stranded DNA Binding Protein (SSB) 以高亲和力协同结合单链 DNA，其与双链 DNA 的结合度不高。与单链 DNA 结合后，SSB 使螺旋二聚体变得不稳定，因此 DNA 聚合酶可以更容易地接触到底物。SSB 参与 DNA 复制并在体内重组。目前它也已用于在电镜下显示单链 DNA。PCR 可通过将这种结合蛋白添加到反应液中而 ... WebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, …
A highly thermostable, homodimeric single-stranded DNA-binding …
WebDec 25, 2013 · INTRODUCTION. Single-stranded (ss) DNA binding (SSB) proteins are ubiquitous and found in bacterial, archaeal and eukaryotic cells, mitochondria, phages and viruses ().SSB proteins bind specifically to ssDNA with high affinity in a sequence-independent manner, protect ssDNA from nucleolytic digestion and prevent intrastrand … WebJan 1, 2005 · Abstract. In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products.The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, … how are black walnuts shelled commercially
Tth-SSB [EG-171] - $0.00 : Excellgen, Recombinant Enzymes
WebTth SSB and Taq SSB have a much lower content of Gly residues than Eco SSB (Table 1). Most of the Gly residues of the thermostable SSB proteins are homologous to conserved Gly in the Eco SSB ... WebWe therefore investigated whether a SSB protein could modify the slippage properties of Pab pols. Thermus thermophilus (Tth) SSB stimulates DNA synthesis of Tth DNA polymerase and the heterologous DNA polymerase from the Archaea P. … WebJan 1, 2006 · The Tth SSB-255 mutant protein was also purified using the same strategy (data not shown). The final products were shown to be 99% pure. Properties of Tth SSB … how many light years to proxima centauri